ゲノム情報科学研究教育機構  アブストラクト
Date March 7, 2011
Speaker Assoc. Prof. Mikita Suyama, Graduate School of Medicine, Kyoto University
Title Searching for regulatory sequences of alternative splicing by using genome sequence alignment and RNA-seq data
Abstract Cis-acting short sequence motifs play important roles in alternative splicing. It is now possible to identify such sequence motifs as conserved sequence patterns in genome sequence alignments. We systematically analyzed the motifs in the neighboring introns of skipped exons (cassette exons) by using comparative analysis of mammalian genome alignments. We identified 11 conserved sequence motifs that might be involved in the regulation of alternative splicing. These motifs are not only significantly overrepresented near alternatively spliced exons, but they also co-occur with each other, thus, forming a network of cis-elements, likely to be the basis for context-dependent regulation. Now I expand the analysis to mutually exclusive splicing, one of the most strictly regulated patterns of alternative splicing, by using RNA-seq data from next-generation sequencing platforms.
「セミナー」に戻る      
 ホーム