アブストラクト
| Date | 11:00-12:00 Apr 23, 2026 |
| Speaker | Adrian-Stefan Andrei Limnological Station, Department of Plant and Microbial Biology, University of Zurich, Switzerland |
| Title | Species-resolved metaproteomics links bacterial identity to in situ proteome allocation |
| Abstract |
A central challenge in microbiome science is connecting microbial identity to realized function: genomic data reveal who is present, yet we often lack quantitative evidence of what those organisms do and how they allocate proteomic resources in natural communities. Metaproteomics can measure expressed proteins, but taxonomic attribution is frequently constrained by incomplete reference databases and peptide sharing among closely related lineages. In this seminar, I will introduce a species-resolved metaproteomics framework that addresses this barrier by pairing proteome-ready hybrid genome references with data-independent acquisition (DIA) proteomics, searched against a minimally redundant, ecosystem-specific protein catalogue. The payoff is species-resolved profiles of proteome allocation that connect genomic potential to realized investment strategies and enable direct tests of genome-based ecological predictions across naturally varying conditions. Finally, I will present a pangenome-resolved extension that leverages single-cell genomes to capture accessory variation in proteomic expression patterns.
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